Differential Cytotoxicity and Gene Expression in Human Liver Carcinoma (HepG2) Cells Exposed to Arsenic Trioxide, and Monosodium Acid Methanearsonate (MSMA)
作者： P. Tchounwou , B. Wilson , A. Abdelghani , A. Ishaque , A. Patlolla
DOI: 10.3390/I3111117
关键词: Hsp70 、 Arsenic 、 Cytotoxicity 、 Biochemistry 、 Cell culture 、 Gadd45 、 Arsenic trioxide 、 Toxicity 、 Viability assay 、 Chemistry
摘要: Research in our laboratory has demonstrated that a trivalent form of arsenic such as trioxide (AT) the ability to cause significant cytotoxicity, and induction number stress genes human liver carcinoma cells (HepG2). However, literature also indicates toxicity depends on its chemical form. To test this hypothesis, we further evaluated cellular molecular responses HepG2 following exposure monosodium acid methanearsonate (MSMA), pentavalent organic arsenic. Cytotoxicity was using MTT-assay for cell viability, while gene profile assay performed measure degree 13 different recombinant lines generated from parental line. experiments yielded LC50 values 11.9 + 2.6 μg/mL AT, 257.3 51.4μg/mL MSMA; indicating AT about 20 times more toxic than MSMA. Exposure MSMA resulted reduction (p < 0.05) induced, compared AT. Upon exposure, only 2 (HMTIIA HSP70) out constructs inductions statistically levels 0.05), 11 (GSTYa, XRE, HMTIIA, c-fos, NF-kBRE, HSP70, p53RE, GADD153, GADD45, GRP78) These results greatly support hypothesis compounds highly their forms; with inorganic forms being potent ones.
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