In vitro assessment of ROS on motility of epididymal sperm of male rat exposed to intraperitoneal administration of nonylphenol
作者： Ansoumane Kourouma , Peng Duan , Hady Keita , Aidogie Osamuyimen , Suqin Qi
DOI: 10.1016/J.APJR.2015.05.002
关键词: Motility 、 Andrology 、 Biology 、 Corn oil 、 Sperm motility 、 Intraperitoneal injection 、 Epididymis 、 Male infertility 、 Malondialdehyde 、 Sperm
摘要: Abstract Objective To explore the mechanism by which nonylphenol (NP) interferes with male infertility through evaluation of its effects on epididymal sperm adult rats. Methods Twenty four Sprague–Dawley (SD) rats were used as donors. Previously administrated NP (0, 2, 10 and 50 mg/kg) body weight respectively in corn oil every forty-eight hours intraperitoneal injection for 30 days. Computer assisted analysis (CASA) was to determine parameters sperm. The morphology examination conducted a high resolution microscope. Results indicated that exposure has no effect weight, while testes weights significantly decreased. showed significant decline percentage motile spermatozoa ( P γ -GT activity H 2 O production malondialdehyde (MDA) increased. Plasma CAT, GSH-Px SOD activities Conclusions This concludes leads oxidative stress Moreover, can disrupt motility alterations morphology.
core.ac.uk
doaj.org
elsevier.com参考文章(63)
J Schwaiger, U Mallow, H Ferling, S Knoerr, Th Braunbeck, W Kalbfus, R.D Negele, How estrogenic is nonylphenol?: A transgenerational study using rainbow trout (Oncorhynchus mykiss) as a test organism Aquatic Toxicology. ,vol. 59, pp. 177- 189 ,(2002) , 10.1016/S0166-445X(01)00248-X
Suresh C. Sikka, Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. Journal of Andrology. ,vol. 25, pp. 5- 18 ,(2004) , 10.1002/J.1939-4640.2004.TB02751.X
Xiao Qin, Zhang Shi-Cui, Hu Jia-Hui, Xu Yu-Yan, Sperm of rosy barb (Puntius conchonius) as an in vitro assay system of nonylphenol cytotoxicity Journal of Environmental Sciences-china. ,vol. 18, pp. 417- 419 ,(2006)
Mi-Sun Choi, Han-Jin Park, Jung-Hwa Oh, Eun-Hee Lee, Se-Myo Park, Seokjoo Yoon, Nonylphenol‐induced apoptotic cell death in mouse TM4 Sertoli cells via the generation of reactive oxygen species and activation of the ERK signaling pathway Journal of Applied Toxicology. ,vol. 34, pp. 628- 636 ,(2014) , 10.1002/JAT.2886
Caroline M. Markey, Jacquelyn C. Labus, Daniel B. Rudolph, Barry T. Hinton, Oxidative Stress Differentially Regulates the Expression of γ‐Glutamyl Transpeptidase mRNAs in the Initial Segment of the Rat Epididymis Journal of Andrology. ,vol. 19, pp. 92- 99 ,(1998) , 10.1002/J.1939-4640.1998.TB02474.X
M. Ahel, J. McEvoy, W. Giger, Bioaccumulation of the lipophilic metabolites of nonionic surfactants in freshwater organisms. Environmental Pollution. ,vol. 79, pp. 243- 248 ,(1993) , 10.1016/0269-7491(93)90096-7
Kanji Yamasaki, Hirokazu Okuda, Tetsuya Takeuchi, Yasushi Minobe, Effects of in utero through lactational exposure to dicyclohexyl phthalate and p,p'-DDE in Sprague-Dawley rats. Toxicology Letters. ,vol. 189, pp. 14- 20 ,(2009) , 10.1016/J.TOXLET.2009.04.023
Beatriz González-Flecha, Oxidant mechanisms in response to ambient air particles Molecular Aspects of Medicine. ,vol. 25, pp. 169- 182 ,(2004) , 10.1016/J.MAM.2004.02.017
Raúl Lagos-Cabré, Ricardo D Moreno, Contribution of environmental pollutants to male infertily: a working model of germ cell apoptosis induced by plasticizers. Biological Research. ,vol. 45, pp. 5- 14 ,(2012) , 10.4067/S0716-97602012000100001
Yi Gong, Xiao D. Han, Nonylphenol-induced oxidative stress and cytotoxicity in testicular Sertoli cells Reproductive Toxicology. ,vol. 22, pp. 623- 630 ,(2006) , 10.1016/J.REPROTOX.2006.04.019