Cytotoxic Effect of As2S3 Nanoparticles on Liver Cancer Cells
作者： Mei Lin , Ziyu Wang , Dongsheng Zhang
关键词: Nanoparticle 、 Analytical chemistry 、 Arsenic 、 Nanobiotechnology 、 Flow cytometry 、 Liver cancer 、 Nuclear chemistry 、 Apoptosis 、 Cytotoxic T cell 、 Chemistry 、 Cancer
摘要: As2S3 is playing an important role in the treatment and research for human cancer, but many limitations exist because of its form. We studied preparation method nanoparticles, a completely new form medicine containing arsenic explored their antitumor effect. nanoparticles were prepared chemically characterized by Transmission electron microscope energy dispersive spectrometry (EDS). Methyl thiazolyl tetrazolium assay, flow cytometry optical electric microscopes performed to examine effect on liver cancer SMMC-7721 cells, compared with that traditional at various concentrations. Average diameter As2S33 was 80 nm. EDS identified they no other components. greatly inhibited proliferation cells apoptosis, concentration- time-dependent manner. The cell survival ratio groups much lower than same concentration incubation time (p < 0.001), apoptotic rate significantly higher. Thus, can produce better As2S3.
sci-hub.se 参考文章(21)
Shigeki Ohtake, Shinji Nakao, Jiebin Zhu, Shinobu Nakamura, Hirokazu Okumura, Arsenic trioxide induces apoptosis in leukemia/lymphoma cell lines via the CD95/CD95L system. Oncology Reports. ,vol. 10, pp. 705- 709 ,(2003) , 10.3892/OR.10.3.705
Manisha P. Desai, Vinod Labhasetwar, Elke Walter, Robert J. Levy, Gordon L. Amidon, The mechanism of uptake of biodegradable microparticles in Caco-2 cells is size dependent. Pharmaceutical Research. ,vol. 14, pp. 1568- 1573 ,(1997) , 10.1023/A:1012126301290
Arun B. Josyula, Gerald S. Poplin, Margaret Kurzius-Spencer, Hannah E. McClellen, Michael J. Kopplin, Stefan Stürup, R. Clark Lantz, Jefferey L. Burgess, Environmental arsenic exposure and sputum metalloproteinase concentrations Environmental Research. ,vol. 102, pp. 283- 290 ,(2006) , 10.1016/J.ENVRES.2006.01.003
Andrew M Evens, Martin S Tallman, Ronald B Gartenhaus, The potential of arsenic trioxide in the treatment of malignant disease: past, present, and future. Leukemia Research. ,vol. 28, pp. 891- 900 ,(2004) , 10.1016/J.LEUKRES.2004.01.011
Somia Bashir, Yukti Sharma, M. Irshad, S. Dutta Gupta, T. D. Dogra, Arsenic-induced cell death in liver and brain of experimental rats. Basic & Clinical Pharmacology & Toxicology. ,vol. 98, pp. 38- 43 ,(2006) , 10.1111/J.1742-7843.2006.PTO_170.X
Yu-Hong Du, Paul Ho, Arsenic compounds induce cytotoxicity and apoptosis in cisplatin-sensitive and -resistant gynecological cancer cell lines. Cancer Chemotherapy and Pharmacology. ,vol. 47, pp. 481- 490 ,(2001) , 10.1007/S002800100278
Teruaki Sakurai, Takami Ohta, Norifumi Tomita, Chikara Kojima, Yukie Hariya, Ayumu Mizukami, Kitao Fujiwara, Evaluation of immunotoxic and immunodisruptive effects of inorganic arsenite on human monocytes/macrophages International Immunopharmacology. ,vol. 4, pp. 1661- 1673 ,(2004) , 10.1016/J.INTIMP.2004.07.027
Ying Deng, Huibi Xu, Kaixun Huang, Xiangliang Yang, Changsheng Xie, Juan Wu, Size effects of realgar particles on apoptosis in a human umbilical vein endothelial cell line: ECV-304. Pharmacological Research. ,vol. 44, pp. 513- 518 ,(2001) , 10.1006/PHRS.2001.0885
T. Bachleitner-Hofmann, M. Kees, H. Gisslinger, Arsenic trioxide: acute promyelocytic leukemia and beyond. Leukemia & Lymphoma. ,vol. 43, pp. 1535- 1540 ,(2002) , 10.1080/1042819021000002857
Ida Stenfeldt Mathiasen, Igor N. Sergeev, Lone Bastholm, Folmer Elling, Anthony W. Norman, Marja Jäättelä, Calcium and calpain as key mediators of apoptosis-like death induced by vitamin D compounds in breast cancer cells Journal of Biological Chemistry. ,vol. 277, pp. 30738- 30745 ,(2002) , 10.1074/JBC.M201558200