Arsenic compounds induce cytotoxicity and apoptosis in cisplatin-sensitive and -resistant gynecological cancer cell lines.

摘要: Purpose: Arsenic compounds have been found to be effective in the treatment of acute promyelocytic leukemia through downregulation bcl-2 expression. Resistant ovarian cancer cells often overexpress or p53 proteins both. We hypothesized that arsenic compounds, such as As2O3 and As2S3, could also active against gynecological cancers resistant conventional chemotherapy. Methods: investigated effects these two vitro on cell lines sensitive (OVCAR, GG, JAM) (CI80-13S) cisplatin (CDDP) human cervical (HeLa) comparison with their fibroblasts (HF). A fluorometric assay based measurements fluorescein diacetate (FDA) was used determine viability. Apoptosis assessed terms morphology, by flow cytometry a DNA fragmentation assay. Results: Treatment each line As2S3 led marked dose-dependent decrease growth. The IC50 indicated significantly greater cytotoxic effect all tested than normal HF. At clinically achievable concentration (2 µM), selectively inhibited growth induced apoptosis CI80-13S, OVCAR HeLa but had no significant apoptotic GG JAM Following 5 µM exhibited inhibition induction apoptosis. Conclusions: (As2O3 As2S3) can inhibit induce at concentrations, indicating cancer.