The longitudinal cerebrospinal fluid metabolomic profile of amyotrophic lateral sclerosis.

摘要: Neurochemical biomarkers are urgently sought in ALS. Metabolomic analysis of cerebrospinal fluid (CSF) using proton nuclear magnetic resonance ((1)H-NMR) spectroscopy is a highly sensitive method capable revealing nervous system cellular pathology. The (1)H-NMR CSF metabolomic signature ALS was longitudinal cohort. Six-monthly serial collection performed patients across range clinical sub-types (n = 41) for up to two years, and healthy controls at single time-point 14). A multivariate statistical approach, partial least squares discriminant analysis, used determine differences between the NMR spectra from controls. Significantly predictive models were found those with one year's interval recruitment second sample. Glucose, lactate, citric acid and, unexpectedly, ethanol discriminating metabolites elevated It concluded that captured associated derangements energy utilization connected ALS, most prominent comparisons longer disease duration. specific identified support concept hypercatabolic state, possibly involving mitochondrial dysfunction specifically. Endogenous may be an unrecognized novel marker neuronal tissue injury