Inflammation pro-resolving potential of 3,4-dihydroxyacetophenone through 15-deoxy-Δ12,14-prostaglandin J2 in murine macrophages
作者： Ping Wu , Li Zhang , Xiaoyan Zhou , Yongsheng Li , Daijuan Zhang
DOI: 10.1016/J.INTIMP.2007.06.008
关键词: Biology 、 Apoptosis 、 Inflammation 、 Eicosanoid 、 DHAP 、 Cyclooxygenase 、 Programmed cell death 、 Pharmacology 、 Biochemistry 、 Prostaglandin 、 Arachidonic acid
摘要: Abstract 3,4-dihydroxyacetophenone (DHAP), an active component isolated from leaves of Tumaodongqing (Ilex Pubescens Hook. Et Arn. Var glaber Chang), is initially used to treat cardiovascular diseases. Previously, we found it had anti-inflammatory effect on macrophages by reducing the production TNF-alpha in vitro. To further determine whether DHAP could influence inflammatory resolution, 15-deoxy-Δ12,14-prostaglandin J2 (15dPGJ2), arachidonic acid metabolite and also crucial pro-resolving mediator inflammation, was chosen as research target. It showed that 10− 5 M resulted obvious increase 15dPGJ2 LPS-activated macrophages. Further, inflammation related cytokines cell apoptosis were studied. We markedly inhibit LPS-stimulated TNF-alpha. However, not change level IL-10 obviously. At same time, LPS-triggered macrophage enhanced significantly. After different kinds cyclooxygenase (COX) inhibitors administrated, effects COX-2 dependent. While, inhibition both COX-1 with indomethacin administration simultaneous reserved enhance at least partly. The mRNA protein detected. expression levels Our results suggest accelerate resolution phase acute though 15dPGJ2, which proved mediate function These are potentially valuable for future use DHAP.
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