Dual effect of 3, 4-dihydroxyacetophenone on LPS-induced apoptosis in RAW264. 7 cells by modulating the production of TNF-α
作者： Wu Ping , Ye Duyun , Zhang Daijuan , Zhang Li , Wan Jingyuan
DOI: 10.1007/BF02873557
关键词: Viability assay 、 Trypan blue 、 Molecular biology 、 Autocrine signalling 、 Biology 、 Tumor necrosis factor alpha 、 Macrophage 、 DHAP 、 Apoptosis 、 Lipopolysaccharide
摘要: To explore the pharmacological effect of 3,4-dihydroxyacetophenone (DHAP) on apoptosis RAW264. 7 macrophage cells and mechanism, were treated with 100 or 500 mg/L lipopolysaccharide (LPS), without 10−5 mol/LDHAP for 24 h. Trypan blue dye exclusion assay was used to assess cell viability. Cell morphological studied flow cytometric used. Tumor necrosis factor-α (TNF-α) level measured by ELISA methods. IϰB protein determined Western blotting. Our results showed that in LPS-stimulated macrophages, DHAP enhanced while significantly inhibited apoptosis. In both groups, increased IκB but decreased TNF-α. It is concluded has dual RAW 264. different concentrations LPS. This may be due inhibition activation NF-κB autocrine production TNFα. study suggests have anti-inflammatory LPS-activated macrophages.
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