Biotype and Ribotype Diversity among Lactobacillus Isolates from the Mouse Ileum

摘要: Summary The present study evaluated the relative diversity of lactobacilli in mouse ileum as defined by conventional phenotypic characteristics versus ribotype patterns. Forty-one Lactobacillus isolates were first presumptively identified using data from carbohydrate fermentation and biochemical tests. Numerical analysis biotype placed a total four species into three major clusters six small clusters. Most typed L. gasseri or fermentum . To further distinguish these isolates, restriction fragment length polymorphism (RFLP) was performed 23S rRNA gene probe to develop ribotypes allow clustering genotypic groups. also revealed main clusters, with identical grouping those generated data, only one exception. Thus, for both generally conserved within A direct microscopic Gram-stain procedure used determine adhesion capacity isolates. varied widely their ability colonize colonic epithelial cell line no apparent relationship between phenotype characteristics.