Peptide-Mediated Gene Transfer into Marine Purple Photosynthetic Bacteria.
作者： Mieko Higuchi-Takeuchi , Takaaki Miyamoto , Choon Pin Foong , Mami Goto , Kumiko Morisaki
DOI: 10.3390/IJMS21228625
关键词: Chemistry 、 Cell-penetrating peptide 、 Biochemistry 、 Plasmid 、 Peptide 、 Bacteria 、 MreB 、 Photosynthetic bacteria 、 Actin 、 Transformation (genetics)
摘要: Use of photosynthetic organisms is one the sustainable ways to produce high-value products. Marine purple bacteria are research focuses as microbial production hosts. Genetic transformation indispensable a biotechnology technique. However, only conjugation has been determined be an applicable method for marine so far. In this study, first time, dual peptide-based combining cell penetrating peptide (CPP), cationic and Tat-derived (dTat-Sar-EED) (containing D-amino acids Tat endosomal escape domain (EED) connected by sarcosine linkers) successfully delivered plasmid DNA into Rhodovulum sulfidophilum, bacterium. The delivery efficiency was greatly improved dTat-Sar-EED. concentrations dTat-Sar-EED, growth stage recovery duration affected delivery. inhibited at 4 °C A22, which inhibitor actin homolog MreB. This suggests that occurred via MreB-mediated energy dependent process. Additionally, peptide-mediated also E. coli cells. Thus, wide range could genetically transformed using novel method.
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