Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam
作者： Lotfi Berguiga , Laura Streppa , Elise Boyer-Provera , Cristina Martinez-Torres , Laurent Schaeffer
DOI: 10.1364/AO.55.001216
关键词: Microscope 、 Total internal reflection 、 Surface plasmon 、 Surface plasmon resonance 、 Optics 、 Plasmon 、 Materials science 、 Numerical aperture 、 Microscopy 、 Interferometry 、 Atomic and Molecular Physics, and Optics
摘要: We report on a fibered high-resolution scanning surface plasmon microscope for long term imaging of living adherent cells. The coupling high numerical aperture objective lens and heterodyne interferometer enhances both the sensitivity stability this microscope, allowing time-lapse recording over several days. diffraction limit is reached with radially polarized illumination beam. Adherence motility C2C12 myoblast cells are followed 50 h, revealing that dynamics these change after 10 h. This enhanced evanescent wave microscopy particularly suited investigating cell adhesion, since it can not only be performed without staining sample but also capture in real time exchange extracellular matrix elements between substrate
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F. SEFAT, M.C.T. DENYER, M. YOUSEFFI, Imaging via widefield surface plasmon resonance microscope for studying bone cell interactions with micropatterned ECM proteins Journal of Microscopy. ,vol. 241, pp. 282- 290 ,(2011) , 10.1111/J.1365-2818.2010.03430.X
Koyo Watanabe, Miyazaki Ryosuke, Goro Terakado, Takashi Okazaki, Kenichi Morigaki, Hiroshi Kano, High resolution imaging of patterned model biological membranes by localized surface plasmon microscopy. Applied Optics. ,vol. 49, pp. 887- 891 ,(2010) , 10.1364/AO.49.000887
Françoise Argoul, Thibault Roland, Audrey Fahys, Lotfi Berguiga, Juan Elezgaray, Uncovering phase maps from surface plasmon resonance images: Towards a sub-wavelength resolution Comptes Rendus Physique. ,vol. 13, pp. 800- 814 ,(2012) , 10.1016/J.CRHY.2012.04.004
Bei Zhang, Suejit Pechprasarn, Jing Zhang, Michael G. Somekh, Confocal surface plasmon microscopy with pupil function engineering Optics Express. ,vol. 20, pp. 7388- 7397 ,(2012) , 10.1364/OE.20.007388
Bei Zhang, Suejit Pechprasarn, Michael G. Somekh, Surface plasmon microscopic sensing with beam profile modulation. Optics Express. ,vol. 20, pp. 28039- 28048 ,(2012) , 10.1364/OE.20.028039
Victor Yashunsky, Vladislav Lirtsman, Michael Golosovsky, Dan Davidov, Benjamin Aroeti, Real-Time Monitoring of Epithelial Cell-Cell and Cell-Substrate Interactions by Infrared Surface Plasmon Spectroscopy Biophysical Journal. ,vol. 99, pp. 4028- 4036 ,(2010) , 10.1016/J.BPJ.2010.10.017
M. Mahadi Abdul Jamil, M.C.T. Denyer, M. Youseffi, S.T. Britland, S. Liu, C.W. See, M.G. Somekh, J. Zhang, Imaging of the cell surface interface using objective coupled widefield surface plasmon microscopy. Journal of Structural Biology. ,vol. 164, pp. 75- 80 ,(2008) , 10.1016/J.JSB.2008.06.005
G. STABLER, M. G. SOMEKH, C. W. SEE, High‐resolution wide‐field surface plasmon microscopy Journal of Microscopy. ,vol. 214, pp. 328- 333 ,(2004) , 10.1111/J.0022-2720.2004.01309.X
Acoustic Microscopy of Elastic Discontinuities Proceedings of The Royal Society A: Mathematical, Physical and Engineering Sciences. ,vol. 393, pp. 171- 183 ,(1984) , 10.1098/RSPA.1984.0052
Cindy K. Miranti, Joan S. Brugge, Sensing the environment: a historical perspective on integrin signal transduction. Nature Cell Biology. ,vol. 4, ,(2002) , 10.1038/NCB0402-E83