Imaging via widefield surface plasmon resonance microscope for studying bone cell interactions with micropatterned ECM proteins

摘要: The widefield surface plasmon resonance microscope has recently been used to monitor label free antibody/antigen binding events and focal contacts in HaCaT cells at high special resolutions. Thus the aim of this study was examine MG63 bone cell attachment alignment microcontact printed extracellular matrix proteins. Collagen, fibronectin laminin were stamp patterned onto glass slides using templates consisting 5-, 10-, 25-, 50- 100-μm-wide repeat grating. seeded 50,000 per 25 cm(2) determined from micrographs taken time-points 2, 5 18 h after seeding. Cells on pattern attached elongated early stages In case collagen laminin, did not adhere readily appeared more rounded until This indicated attach mostly via specific integrins. aligned well fibronectin-patterned cover slips especially patterns, although position themselves middle each fibronectin-coated region, but instead small features associated with edges regions. Patterned un-patterned also had quite different morphologies. a morphology lengths 35 μm, whereas direction 50-70 μm. imaging that surfaces which evenly distributed around periphery cell. However, substrates organized most their along distal edge patterns. suggests interaction between induces reorganization such region guided by is relatively loosely coupled culture substrate, positioned away tightly substrate. turn guidance necessarily enhanced substrate coupling cue, may be decreased cue. Such an arrangement influence cytoplasmic streaming as modulate extension. Verification finding required; response cue unexpected because it believed cluster