Neuropeptides and adrenocortical proliferation in vitro.

摘要: Vasoactive intestinal peptide (VIP) and Neuropeptide Y (NPY) are localised in the capsule zona glomerulosa region of adrenal cortex, where they play an important role regulating steroidogenesis blood flow. This study investigates effect these neuropeptides on adrenocortical cellular proliferation vitro. Capsular/glomerulosa innerzone/medulla preparations were either stimulated acutely with NPY or VIP (both 10–6 M) for up to 2 hours 24 hours, four eight days vitro eagles MEM (3.4 mM K+). DNA synthesis was determined using immunocytochemistry through incorporation thymidine analogue 5-bromo-2′-deoxyridine (BrdU, 20 mg/mL). Phosphorylation mitogen activated protein kinase ERK1/2 assessed by western blotting. Both (10–6 M) treatment caused increase after culture. Acute ERK1 2 phosphorylation (p < 0.01) capsular/zona glo...