Matrix metalloproteinase 9 promoter activity is induced coincident with invasion during tumor progression.

摘要: Matrix metalloproteinase 9 (MMP-9, also known as gelatinase B or 92-kd Type IV collagenase) is overexpressed in many human and murine cancers. We induced carcinomas mice carrying a transgene that links the MMP-9 promoter to reporter β-galactosidase so activation of would be indicated by β-galactosidase. Mammary were mating transgenic with for mammary tumor virus linked polyoma middle T antigen, leads rapid development tumors female mice. None hyperplastic glands none situ expressed However, all invasive had evidence expression. In addition breast carcinomas, malignant teratoma papillary adenocarcinoma pelvic region male arose positive. skin 7, 12-dimethylbenz[a]anthracene (DMBA) treatment followed phorbol 12 myristate 13-acetate (TPA). papillomas showed any expression, but expression was seen carcinoma. Although normal epithelial cells did not express β-galactosidase, we find staining few at duct sebaceous gland base hair follicles. The lead alveolar macrophages, confirming additional upstream sequences are required macrophages. These experiments have revealed activity coincident invasion during progression. Furthermore, this indicates more proximal elements sufficient transcription