Is the loss of pRb essential for the mouse skin carcinogenesis

摘要: The pRb pathway is inactivated in most, if not all, human and mouse tumors, including skin tumors.However, a relatively low frequency of Rb gene alterations found. embryonic lethality pRbdeficientanimals restricts the analysis these mice to midgestation precludes rolesof cancer models. To solve this problem, we used Cre/LoxP technology induce thetissue-specific deletion pRb. In epidermis, leads altered proliferation differentiationbut do development spontaneous tumors. gain insight thepossible roles carcinogenesis, have performed chemical tumorigenesisexperiments bearing epidermal-specific inactivation gene. Unexpectedly, developfewer smaller tumors than control animals, but showing increased malignant conversion squamouscell carcinomas. Detailed biochemical demonstrates that, absence pRb, multiple pathways...