An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi

作者: Jeffrey N. Davidson , Maureen R. Hanson , Lawrence Bogorad

DOI: 10.1007/BF00272177

关键词: MutantGeneticsChlamydomonasGeneChloroplastRibosomal proteinLocus (genetics)Structural geneGel electrophoresisBiology

摘要: A unified biochemical and genetic study has led to the location of gene for erythromycin resistance in 4 mutants Chalamydomonas reinhardi identification chloroplast ribosomal protein altered these strains. Tetrad analyses crosses involving Mendelian ery-M1a, ery-M1b, ery-M1c, ery-M1d indicate that they are closely-linked alleles which map nuclear linkage group XI. The proteins 52s subunits wild-type ery-M1 were compared by one-and two-dimensional gel electrophoresis. Protein LC6 from differs net charge at pH 5 homologous component found each mutants. ery-M1b is also 30% smaller molecular weight than protein. variety forms observed allelic nature determinants strongly support view locus structural LC6.

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