Antisense down-regulation of strawberry endo-β-(1,4)-glucanase genes does not prevent fruit softening during ripening
作者： Xavier Palomer , Imma Llop-Tous , Miquel Vendrell , Frans A. Krens , Jan G. Schaart
DOI: 10.1016/J.PLANTSCI.2006.06.019
关键词: Fragaria 、 Ripening 、 Glucanase 、 Gene 、 Horticulture 、 Diminution 、 Biology 、 Transgene 、 Softening 、 Biochemistry 、 Pectate lyase
摘要: Abstract Strawberry (Fragaria × ananassa Duch.) fruit softening during ripening is associated with the overlapping presence of two divergent endo-β-(1,4)-glucanases (EC 3.2.1.4; EGases), Cel1 and Cel2. Antisense down-regulation both genes was performed to assess precise role these on strawberry softening. Constant cel1 expression throughout obtained, which accompanied by reduced protein accumulation. However, diminution level together a reduction total EGase activity 40% control did not affect firmness, thus suggesting that major determinant ripening. On other hand, no significant Cel2 accumulation found in any transgenic or Cel1/Cel2 double-transgenic lines obtained. The difficulties encountered yield line cel2 suggest this gene might be playing pivotal development prior ripening, accounting for lack observed.
narcis.nl
elsevier.com参考文章(34)
N. Medina-Escobar, J. Cárdenas, E. Moyano, J.L. Caballero, J. Muñoz-Blanco, Cloning, molecular characterization and expression pattern of a strawberry ripening-specific cDNA with sequence homology to pectate lyase from higher plants Plant Molecular Biology. ,vol. 34, pp. 867- 877 ,(1997) , 10.1023/A:1005847326319
Mary L. Durbin, Lowell N. Lewis, Cellulases in Phaseolus vulgaris Methods in Enzymology. ,vol. 160, pp. 342- 351 ,(1988) , 10.1016/0076-6879(88)60137-6
Mark H. Harpster, David A. Brummell, Pamela Dunsmuir, Suppression of a ripening-related endo-1,4-β-glucanase in transgenic pepper fruit does not prevent depolymerization of cell wall polysaccharides during ripening Plant Molecular Biology. ,vol. 50, pp. 345- 355 ,(2002) , 10.1023/A:1019856929126
David A. Brummell, Bradford D. Hall, Alan B. Bennett, Antisense suppression of tomato endo-1,4-beta-glucanase Cel2 mRNA accumulation increases the force required to break fruit abscission zones but does not affect fruit softening. Plant Molecular Biology. ,vol. 40, pp. 615- 622 ,(1999) , 10.1023/A:1006269031452
Livio Trainotti, Silvia Spolaore, Anna Pavanello, Barbara Baldan, Giorgio Casadoro, A novel E-type endo-beta-1,4-glucanase with a putative cellulose-binding domain is highly expressed in ripening strawberry fruits. Plant Molecular Biology. ,vol. 40, pp. 323- 332 ,(1999) , 10.1023/A:1006299821980
Karl Erik Almin, Karl-Erik Eriksson, Christer Jansson, Enzymic degradation of polymers. II. Viscometric determination of cellulase activity in absolute terms. Biochimica et Biophysica Acta. ,vol. 139, pp. 248- 253 ,(1967) , 10.1016/0005-2744(67)90029-0
Mark H. Harpster, Debra M. Dawson, Donald J. Nevins, Pamela Dunsmuir, David A. Brummell, Constitutive overexpression of a ripening-related pepper endo-1,4-β-glucanase in transgenic tomato fruit does not increase xyloglucan depolymerization or fruit softening Plant Molecular Biology. ,vol. 50, pp. 357- 369 ,(2002) , 10.1023/A:1019888129013
L. C. BEADLE, SOCIETY FOR EXPERIMENTAL BIOLOGY Nature. ,vol. 159, pp. 718- 719 ,(1947) , 10.1038/159718A0
J. Schaart, E. Salentijn, F. Krens, Tissue-specific expression of the β-glucuronidase reporter gene in transgenic strawberry (Fragaria · ananassa) plants Plant Cell Reports. ,vol. 21, pp. 313- 319 ,(2002) , 10.1007/S00299-002-0514-4
Xavier Palomer, Eva Domı́nguez-Puigjaner, Miquel Vendrell, Imma Llop-Tous, Study of the strawberry Cel1 endo-β-(1,4)-glucanase protein accumulation and characterization of its in vitro activity by heterologous expression in Pichia pastoris Plant Science. ,vol. 167, pp. 509- 518 ,(2004) , 10.1016/J.PLANTSCI.2004.04.017