In vitro biological activities of the E6 and E7 genes vary among human papillomaviruses of different oncogenic potential.

摘要: Human papillomavirus type 16 (HPV-16) and HPV-18 are often detected in cervical carcinomas, while HPV-6, although frequently present benign genital lesions, is only rarely cancers of the cervix. Therefore, infections with HPV-16 considered high risk infection HPV-6 low risk. We found, by using a heterologous promoter system, that expression E7 transforming protein differs between high- low-risk HPVs. In high-risk HPV-16, expressed from constructs containing complete upstream E6 open reading frame. contrast, was efficiently translated when deleted. By clones which coding regions E7s were preceded identical leader sequences, we found ability gene products to induce anchorage-independent growth rodent fibroblasts correlated directly oncogenic association HPV types. an immortalization assay normal human keratinocytes requires complementation E7, both could complement corresponding HPV-16. However, neither nor able substitute for or HPV-18. Our results suggest multiple factors, including lower intrinsic biological activity differences regulation their expression, account comparison HPV-18, vitro assays. These same factors may, part, apparent difference potential these viruses.