Culture‐expanded mesenchymal stem cell sheets enhance extraction‐site alveolar bone growth: An animal study

摘要: BACKGROUND AND OBJECTIVES Impaired bone formation of the buccal alveolar plate after tooth extraction during adolescence increases difficulty future implant restoration. This study was undertaken to assess feasibility and efficacy transplanting autogenous scaffold-free culture-expanded mesenchymal stem cell (MSC) sheets surface stimulate local growth. MATERIAL METHODS Mandibular marrow aspirated from 3-month-old pigs (n = 5), which MSCs were isolated culture expanded. Triple-layer MSC then fabricated using temperature-responsive tissue plates. One month aspirations, same underwent bilateral mandibular primary molars, immediately followed by transplantation 3 triple-layer on subperiosteal 1 randomly chosen side. The contralateral side (control) periosteal reflection surgery without receiving sheet transplantation. Six weeks later, animals killed specimens both sides harvested for radiographic histological analysis. Buccal thickness, mineral density (TMD), apposition volume fraction (BV/TV) quantified compared between control paired t-tests. RESULTS reliably majority cells remained vital before thickness tended increase with (P = .18), 4 5 showing an average 1.82 ± 0.73 mm thicker than After being normalized TMD intracortical bone, cortical 0.5-fold higher (P < .05). Likewise, BV/TV measurements region also 0.4-fold (P < .05) region. Mineral not different 2 sides. CONCLUSION marrow-derived can be into onto at tooth-extraction site may density.