作者: Peter F. Stanbury , Allan Whitaker , Stephen J. Hall
DOI: 10.1016/B978-0-08-099953-1.00012-0
关键词: Gene expression 、 Fusion protein 、 Heterologous expression 、 Expression vector 、 Cloning vector 、 Heterologous 、 Computational biology 、 Chinese hamster ovary cell 、 Context (language use) 、 Molecular biology 、 Biology
摘要: This chapter considers the production of heterologous proteins by Escherichia coli , yeasts, and mammalian cell cultures. The reader is introduced to basic process gene cloning advantages limitations each system. issues expression vector structure, expression, secretion are addressed for systems their ability perform posttranslational modifications compared discussed. Both genetic physiological manipulation host considered in context design process.