The production of heterologous proteins

作者: Peter F. Stanbury , Allan Whitaker , Stephen J. Hall

DOI: 10.1016/B978-0-08-099953-1.00012-0

关键词: Gene expressionFusion proteinHeterologous expressionExpression vectorCloning vectorHeterologousComputational biologyChinese hamster ovary cellContext (language use)Molecular biologyBiology

摘要: This chapter considers the production of heterologous proteins by Escherichia coli , yeasts, and mammalian cell cultures. The reader is introduced to basic process gene cloning advantages limitations each system. issues expression vector structure, expression, secretion are addressed for systems their ability perform posttranslational modifications compared discussed. Both genetic physiological manipulation host considered in context design process.

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