Transient Recombinant Protein Expression in Mammalian Cells

摘要: Transient gene expression has evolved into an attractive technology for the rapid production of milligram to gram amounts recombinant proteins. This review describes different methods introducing foreign DNA suitable mammalian cells with either viral or non-viral vectors. Particular emphasis is given transient transfection which represents meanwhile most prominent variant due recent progress in resulting protein productivity. Non-viral protocols are always based on use specific reagents application electroporation device. The corresponding compared regard their scale-up potential, also consideration potential costs. underlying cellular pathways plasmid incorporation, cytoplasmic release and translocation nucleus important details understand principle further improve technology. Problems associated at a larger scale addressed. In particular, requirement cell culture media conditions complex preparation (if necessary), process itself high titer need be harmonized. Strategies productivity by increasing cell-specific output and/or sustaining phase itemized as well. can accomplished enabling perform episomal replication, co-transfection other plasmids, altering metabolism, temperature reduction, supplementation enhancers combinations thereof. A number examples successful applications pilot provided.