The Carotenogenesis Pathway via the Isoprenoid-β-carotene Interference Approach in a New Strain of Dunaliella salina Isolated from Baja California Mexico

摘要: D. salina is one of the recognized natural sources to produce β-carotene, and an useful model for studying role inhibitors enhancers carotenogenesis. However there little information in regarding whether isoprenoid substrate can be influenced by stress factors (carotenogenic) or selective which turn may further contribute elucidate early steps carotenogenesis biosynthesis β-carotene. In this study,Dunaliella (BC02) isolated from La Salina BC Mexico, was subjected method isoprenoids-β-carotene interference order promote interruption accumulation programmed carotenoids. When Carotenogenic non-carotenogenic cells BC02 were grown under photoautotrophicgrowth conditions presence 200 µM fosmidomycin, synthesis β-carotene interrupted after two days cultured cells. This result indirect consequence inhibition isoprenoids activity recombinant DXR enzyme thereby preventing conversionof 1-deoxy-D-xylulose 5-phosphate (DXP) 2-C-methyl-D-erythritol (MEP) consequently interrupts salina. The effect at level proteins RNA not evident. Mevinolin treated exhibited levels very similar those control cell cultures indicating that mevinolin pursued any action had no HMG-CoA reductase, key Ac/MVA pathway.