Riboflavin 3'- and 5'-sulfate, two novel flavins accumulating in the roots of iron-deficient sugar beet (Beta vulgaris).

摘要: Roots from iron-deficient sugar beet grown in the presence of calcium carbonate exhibit a yellow color and autofluorescence typical flavin-like compounds, whereas roots control, iron-sufficient plants exhibited no extremely low autofluorescence. The two major flavins whose accumulation is induced by iron deficiency have been shown to be different riboflavin, FMN, FAD reversed-phase high performance liquid chromatography. These flavins, accounting for 82 15% total flavin concentration deficient roots, unequivocally riboflavin 3‘-sulfate 5‘-sulfate, respectively, electrospray-mass spectrometry, inductively coupled plasma emission spectroscopy, infrared 1H nuclear magnetic resonance. sulfates not found previously biological systems. localization similar, but identical, that reductase activity. has estimated root extracts at least 1 mM. We hypothesize, based on similar reduction, may an integral part turbo iron-reducing system roots.