CHANGES IN SUGAR BEET LEAF PLASMA MEMBRANE FE(III)-CHELATE REDUCTASE ACTIVITIES MEDIATED BY FE-DEFICIENCY, ASSAY BUFFER COMPOSITION, ANAEROBIOSIS AND THE PRESENCE OF FLAVINS
作者： E. B. Gonz�lez-Vallejo , S. Sus�n , A. Abad�a , J. Abad�a
DOI: 10.1007/BF01279306
关键词:
摘要: Different assay conditions induce changes in the ferric chelate reductase activities of leaf plasma membrane preparations from Fe-deficient and Fe-sufficient sugar beet. With an apoplasttype medium did not change significantly when Fe(III)-EDTA was substrate. However, with citrate as substrate, effect depended on citrateto-Fe ratio. When citrate-to-Fe ratio 20 ∶ 1, effects were practically unappreciable. a lower 5 1 apoplast-type than standard medium. Our data also indicate that anaerobiosis during facilitates reduction malate by preparations. Anaerobiosis increased approximately 50% 70–90% over values obtained aerobic conditions. increase activity significant. We have tested riboflavin, flavin adenine dinucleotide, mononucleotide activities. The presence flavins generally control plants. Increases moderate (lower twofold). These increases occurred Fe(III)-citrate substrates.
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