The pH Requirement for in Vivo Activity of the Iron-Deficiency-Induced "Turbo" Ferric Chelate Reductase (A Comparison of the Iron-Deficiency-Induced Iron Reductase Activities of Intact Plants and Isolated Plasma Membrane Fractions in Sugar Beet).
作者： S. Susin , A. Abadia , J. A. Gonzalez-Reyes , J. J. Lucena , J. Abadia
DOI: 10.1104/PP.110.1.111
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摘要: The characteristics of the Fe reduction mechanisms induced by deficiency have been studied in intact plants Beta vulgaris and purified plasma membrane vesicles from same plants. In Fe-deficient vivo Fe(III)-ethylenediaminetetraacetic complex [Fe(III)-EDTA] reductase activity increased over control values 10 to 20 times when assayed at a pH 6.0 or below ("turbo" reductase) but only 2 4 6.5 above. Fe(III)-EDTA root preparations 3.5 controls, irrespective assay pH. Km for ferric chelate was approximately 510 240 [mu]M ranges 4.5 8.0, respectively. isolated 200 [mu]M. Therefore, turbo low appears be different constitutive reductase.
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