Production of an amylase-degrading raw starch by Gibberella pulicaris.

作者: Yetti Marlida , Nazamid Saari , Son Radu , Fatimah Abu Bakar , None

DOI: 10.1023/A:1005685118614

关键词: Food scienceEnzyme assayHydrolysisBiologyBotanyFermentationAmylaseStarchGibberella pulicarisPotato starchEnzyme

摘要: An endophytic fungus, Gibberella pulicaris, produced an amylase which degraded raw starches from cereals and other crops including potato, sago, tapioca, corn, wheat rice starch. In each case, glucose was the main product. Among used, potato starch gave highest enzyme activity (85 units mg−1 protein) lowest (49 protein). The production (260 achieved when concentration of increased to 60 g l−1. Optimum hydrolysis at 40 °C pH 5.5.

参考文章(8)
Bartholomew N Okolo, Lewis I Ezeogu, Charles N Mba, None, Production of raw starch digesting amylase by Aspergillus niger grown on native starch sources Journal of the Science of Food and Agriculture. ,vol. 69, pp. 109- 115 ,(1995) , 10.1002/JSFA.2740690117
G. L. Miller, Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Analytical Chemistry. ,vol. 31, pp. 426- 428 ,(1959) , 10.1021/AC60147A030
Roch-chui Yu, Y.D. Hang, Purification and characterization of a glucoamylase from Rhizopus oryzae Food Chemistry. ,vol. 40, pp. 301- 308 ,(1991) , 10.1016/0308-8146(91)90114-4