Relative rates of divergence of spacer and gene sequences within the rDNA region of species in the Triticeae: Implications for the maintenance of homogeneity of a repeated gene family.

作者: R Appels , J Dvořák , None

DOI: 10.1007/BF00303907

关键词: Spacer DNARibosomal RNABiologyTriticeae18S ribosomal RNAGeneticsGene familyInternal transcribed spacerGene conversionGene

摘要: The relative rates of divergence 11 regions the wheat rDNA cloned in pTA250 were estimated by measuring sequence change 6 Triticum species. Tm analysis 32P probes synthesized from and hybridized to DNA species provided an estimate T. aestivum. results revealed a region 1.2 kb preceding 18S rRNA gene which was more conserved than rest spacer. In addition transcribed spacer between 26S genes shown be poorly-conserved; genes, as expected, highly conserved. A model proposes RNA co-factor conversion is suggested account for observations.

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