Expression of common housekeeping genes is affected by disease in human hepatitis C virus‐infected liver
作者： Mario Congiu , John L. Slavin , Paul V. Desmond
DOI: 10.1111/J.1478-3231.2010.02374.X
关键词: Liver disease 、 Biology 、 Hepatitis C virus 、 Gene expression profiling 、 Hepatitis 、 Molecular biology 、 Housekeeping gene 、 Liver biopsy 、 Fatty liver 、 Hepatitis C
摘要: Background: Comparative gene expression is commonly determined with reference to the of a housekeeping (HKG), level which assumed be unregulated. There are little data date on effect disease classic HKGs in hepatitis C virus (HCV)-infected human liver. Aims: To identity stable across wide spectrum HCV-infected liver. Methods: β-Actin, hypoxanthine phosphoribosyltransferase 1 (HPRT1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), splicing factor arginine/serine-rich 4, β-glucuronidase and 18S ribosomal RNA (18S rRNA) were measured by real-time polymerase chain reaction liver biopsy tissue. Samples categorised for inflammation, fibrosis steatosis, allocated into groups mild or severe disease. Values analysed using Spearman's rank correlation, NormFinder, BestKeeper geNorm programs. Results: All genes performed well samples patients low activity, but HPRT1, β-actin, GAPDH rRNA ranked poorly inflammation. Conclusions: Our results indicate that affects common 4 most from this group studies liver.
sci-hub.se 参考文章(17)
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Takeya Tsutsumi, Tetsuro Suzuki, Takashi Shimoike, Ryosuke Suzuki, Kyoji Moriya, Yoshizumi Shintani, Hajime Fujie, Yoshiharu Matsuura, Kazuhiko Koike, Tatsuo Miyamura, Interaction of hepatitis C virus core protein with retinoid X receptor α modulates its transcriptional activity Hepatology. ,vol. 35, pp. 937- 946 ,(2002) , 10.1053/JHEP.2002.32470
Kung Ahn, Jae-Won Huh, Sang-Je Park, Dae-Soo Kim, Hong-Seok Ha, Yun-Ji Kim, Ja-Rang Lee, Kyu-Tae Chang, Heui-Soo Kim, None, Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey (Macaca mulatta) tissues BMC Molecular Biology. ,vol. 9, pp. 78- 78 ,(2008) , 10.1186/1471-2199-9-78
N.A. Janovick-Guretzky, H.M. Dann, D.B. Carlson, M.R. Murphy, J.J. Loor, J.K. Drackley, Housekeeping gene expression in bovine liver is affected by physiological state, feed intake, and dietary treatment. Journal of Dairy Science. ,vol. 90, pp. 2246- 2252 ,(2007) , 10.3168/JDS.2006-640
Gulam Waris, Daniel Jeffery Felmlee, Francesco Negro, Aleem Siddiqui, None, Hepatitis C Virus Induces Proteolytic Cleavage of Sterol Regulatory Element Binding Proteins and Stimulates Their Phosphorylation via Oxidative Stress Journal of Virology. ,vol. 81, pp. 8122- 8130 ,(2007) , 10.1128/JVI.00125-07
Nicholas Silver, Emanuele Cotroneo, Gordon Proctor, Samira Osailan, Katherine L Paterson, Guy H Carpenter, Selection of housekeeping genes for gene expression studies in the adult rat submandibular gland under normal, inflamed, atrophic and regenerative states BMC Molecular Biology. ,vol. 9, pp. 64- 64 ,(2008) , 10.1186/1471-2199-9-64
Ewart W Kuijk, Leonie du Puy, Helena TA van Tol, Henk P Haagsman, Ben Colenbrander, Bernard AJ Roelen, Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos BMC Developmental Biology. ,vol. 7, pp. 58- 58 ,(2007) , 10.1186/1471-213X-7-58
P Bedossa, T Poynard, An algorithm for the grading of activity in chronic hepatitis C Hepatology. ,vol. 24, pp. 289- 293 ,(1996) , 10.1002/HEP.510240201
Ashish Mogal, Sarki A. Abdulkadir, Effects of Histone Deacetylase Inhibitor (HDACi); Trichostatin-A (TSA) on the expression of housekeeping genes Molecular and Cellular Probes. ,vol. 20, pp. 81- 86 ,(2006) , 10.1016/J.MCP.2005.09.008
Gregory L. Armstrong, Annemarie Wasley, Edgar P. Simard, Geraldine M. McQuillan, Wendi L. Kuhnert, Miriam J. Alter, The prevalence of hepatitis C virus infection in the United States, 1999 through 2002. Annals of Internal Medicine. ,vol. 144, pp. 705- 714 ,(2006) , 10.7326/0003-4819-144-10-200605160-00004