The housekeeping gene YWHAZ remains stable in a model of developmentally primed non alcoholic fatty liver disease

摘要: Background Non-alcoholic fatty liver disease (NAFLD) is the most common form of chronic in Western society. Comparative gene expression studies are beginning to elucidate molecular mechanisms underlying NAFLD progression. We have previously shown that high fat diets during early life can prime non-alcoholic steatohepatitis (NASH) adulthood, through lipogenesis elevation. To generate accurate results such studies, appropriate housekeeping genes (HKG) which unaffected by processes, used for data normalisation. However, there little existing show effects on HKG expression. Aims To identify a mouse model developmentally primed and NASH, maintains stability. Methods We determined stability six candidates (GAPDH, YWHAZ, B2M, EIF4A2, ACTB CYC1) both day night, using geNORM qBasePlus software. Results HKG differed across dietary groups time day. In majority treatment points stable was YWHAZ. Following diet interventions CYC1 became notably unstable. Overall effect NASH maintain but destabilise EIF4A2. Conclusions Our clearly shows affected severity sampling, highlighting importance suitable selection. For comparative investigating we would recommend use YWHAZ as robust, stably expressed HKG.