Mono-PEGylation of Alpha-MMC and MAP30 from Momordica charantia L.: Production, Identification and Anti-Tumor Activity

作者: Yun Sun , Fenghui Sun , Jianlong Li , Minlu Wu , Xiang Fan

DOI: 10.3390/MOLECULES21111457

关键词: LysineElectrophoresisIn vivoChemistryChromatographyBiochemistryPEGylationPEG ratioIsoelectric focusingPolyacrylamide gel electrophoresisMomordica

摘要: PEGylation is a well-established and effective strategy to decrease immunogenicity, which can increase the stability and in vivo half-life time. However, the generation of multi-site …

参考文章(23)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Jonathan Dozier, Mark Distefano, Site-Specific PEGylation of Therapeutic Proteins. International Journal of Molecular Sciences. ,vol. 16, pp. 25831- 25864 ,(2015) , 10.3390/IJMS161025831
Xiaoxiao Bian, Fubing Shen, Yiwen Chen, Baoning Wang, Mian Deng, Yanfa Meng, PEGylation of alpha-momorcharin: synthesis and characterization of novel anti-tumor conjugates with therapeutic potential Biotechnology Letters. ,vol. 32, pp. 883- 890 ,(2010) , 10.1007/S10529-010-0242-8
Xingchuan Yao, Juan Li, Nianhua Deng, Shurui Wang, Yanfa Meng, Fubing Shen, Immunoaffinity purification of α-momorcharin from bitter melon seeds (Momordica charantia) Journal of Separation Science. ,vol. 34, pp. 3092- 3098 ,(2011) , 10.1002/JSSC.201100235
Qiang Lv, Xu-Zhong Yang, Long-Yun Fu, Yv-Ting Lu, Yan-Hua Lu, Jian Zhao, Fu-Jun Wang, None, Recombinant expression and purification of a MAP30-cell penetrating peptide fusion protein with higher anti-tumor bioactivity Protein Expression and Purification. ,vol. 111, pp. 9- 17 ,(2015) , 10.1016/J.PEP.2015.03.008
Inderdeep Kaur, R. C. Gupta, Munish Puri, Ribosome inactivating proteins from plants inhibiting viruses Virologica Sinica. ,vol. 26, pp. 357- 365 ,(2011) , 10.1007/S12250-011-3223-8
Fiorenzo Stirpe, Luigi Barbieri, Maria Giulia Battelli, Marco Soria, Douglas A. Lappi, Ribosome-inactivating proteins from plants: present status and future prospects. Nature Biotechnology. ,vol. 10, pp. 405- 412 ,(1992) , 10.1038/NBT0492-405
Jana Budilová, Jirí Pazourek, Pavel Krásenský, Jan Pospíchal, Continuous mode of operation for large volume dosing in analytical carrier ampholyte-free isoelectric focusing of proteins applied to off-line detection of fractions. Journal of Separation Science. ,vol. 29, pp. 1613- 1621 ,(2006) , 10.1002/JSSC.200500458
Hiroshi Ishihara, Current status and prospects of polyethyleneglycol-modified medicines. Biological & Pharmaceutical Bulletin. ,vol. 36, pp. 883- 888 ,(2013) , 10.1248/BPB.B13-00087