In vitro study examining the effect of sub-lethal QS 755 nm lasers on the expression of p16INK4a on melanoma cell lines.

摘要: Background and Objectives Q-switched lasers had been used in the treatment of lentigo maligna but their role remains controversial. While previous studies have addressed change adhesion molecule expression after sub-lethal laser damage, no study has impact damage at a molecular level. The p16 gene proposed as candidate for melanoma. Our objective is to examine effect on melanoma cell lines. Study Design/Materials Methods Three human lines—HTB 66, Sk-mel-24 (HTB 71), G361—were irradiated by Q-switched 755 nm Alexandrite fluencies that ranged from 0.85 2.0 J/cm2. HTB 66 was only line with significant p16INK4a while other two cells lines were negative served control. Protein mRNA assessed flow cytometry RT-PCR, respectively. Results The level protein increased significantly irradiation compared non-irradiated cells. did not p16INK4a-negative (Sk-mel-24 G361). However, there slight increase percentage G0/G1 phase cells. Conclusions Sub-lethal could DNA leading an expression, such would be particularly undesirable patients mutation. Further are warranted inducing Lasers Surg. Med. 32:88–93, 2003. © 2003 Wiley-Liss, Inc.