Synthesis of Pesticide-Specific Single-Chain FV Utilizing the Recombinant Phage Antibody System (RPAS, Pharmacia). A Detailed Protocol
作者： Karl Kramer
DOI: 10.1080/00032719808001834
关键词: Computational biology 、 Chemistry 、 Phage antibody 、 Protocol (science) 、 Single-Chain Fv 、 Molecular biology 、 Phage display 、 Rab 、 Recombinant DNA
摘要: Abstract Recombinant antibody (Rab) technology is expected to play an essential role in immunological research and basically technologies which are utilizing the benefits of antibodies as a central component. Although commercial kits already available for synthesis Rab, Rab approach far away from being considered standard method. This mainly due fact that complex procedure frequently needs technical refinement order be adapted individual experimental conditions goals. In contrast, manuals, supplied by manufacturers, appropriately designed guide general application strategy. Here, we focus exclusively on Phage Antibody System (RPAS, Pharmacia). The RPAS kit provide tool phage-displayed soluble Rab. work adressed applicants RPAS, encourage them modify manufacturers manual, especially if problems arise at particular steps when following t...
sci-hub.se 参考文章(20)
C. R. Newton, PCR : essential data J. Wiley. ,(1995)
Elvin A. Kabat, Sequences of proteins of immunological interest National Institutes of Health. ,(1991)
Robert E. Hawkins, Stephen J. Russell, Greg Winter, Selection of phage antibodies by binding affinity. Mimicking affinity maturation. Journal of Molecular Biology. ,vol. 226, pp. 889- 896 ,(1992) , 10.1016/0022-2836(92)90639-2
The polymerase chain reaction Química : actualidad y futuro. ,vol. 4, pp. 17- 24 ,(1994) , 10.1007/978-1-4612-0257-8
James D. Marks, Hennie R. Hoogenboom, Timothy P. Bonnert, John McCafferty, Andrew D. Griffiths, Greg Winter, By-passing immunization: Human antibodies from V-gene libraries displayed on phage Journal of Molecular Biology. ,vol. 222, pp. 581- 597 ,(1991) , 10.1016/0022-2836(91)90498-U
P. Quirke, G. R. Taylor, M. J. McPherson, PCR: a practical approach. IRL Press at Oxford University Press. ,(1991)
Dominic De Bellis, Ira Schwartz, Regulated expression of foreign genes fused to lac: control by glucose levels in growth medium Nucleic Acids Research. ,vol. 18, pp. 1311- 1311 ,(1990) , 10.1093/NAR/18.5.1311
J. Scott, G. Smith, Searching for Peptide Ligands With an Epitope Library Science. ,vol. 249, pp. 386- 390 ,(1990) , 10.1126/SCIENCE.1696028
M Better, C. Chang, R. Robinson, A. Horwitz, Escherichia coli secretion of an active chimeric antibody fragment Science. ,vol. 240, pp. 1041- 1043 ,(1988) , 10.1126/SCIENCE.3285471
R. Orlandi, D. H. Gussow, P. T. Jones, G. Winter, Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 3833- 3837 ,(1989) , 10.1073/PNAS.86.10.3833