Enzyme and Immunoassays

摘要: The article contains sections titled: 1. Enzymatic Analysis Methods 1.1. Introduction 1.2. Enzymes: Basic Kinetics 1.3. Enzyme Assays: Practical Aspects 1.3.1. General Considerations 1.3.2. Spectrometric Methods 1.3.3. Other Methods 1.3.4. Effects of pH, Buffer, Composition, and Temperature 1.3.5. Sources Activity Enzymes 1.4. Enzyme Determination Substrates 1.4.1. General Considerations 1.4.2. Determination Glucose 1.4.3. Determination Ethanol 1.5. Enzyme Inhibitors 1.5.1. Inhibitors Cholinesterase Enzymes 1.5.2. Other Inhibition Methods 1.6. Enzyme Activators 1.6.1. Determination Metal Ions by Metalloenzymes 1.6.2. Other Activator Analyses 1.7. Immobilized Enzymes 1.7.1. Introduction 1.7.2. Properties Immobilized Enzymes 1.7.3. Application Enzymes 2. Immunoassays in Analytical Chemistry 2.1. Introduction 2.2. Polyclonal, Monoclonal, Recombinant Antibodies 2.3. Sample Conditioning 2.4. Immunoassays 2.4.1. Radioimmunoassay 2.4.1.1. Isotopic Dilution Radioimmunoassay 2.4.1.2. Immunoradiometric Assay (IRMA) 2.4.2. Nonisotopic Homogeneous Immunoassay 2.4.2.1. Latex Particle Agglutination Immunoassay 2.4.2.2. Enzyme-Multiplied Immunoassay Technique (EMIT) 2.4.2.3. Apoenzyme Reconstitution System (ARIS) 2.4.2.4. Fluorophore-Labeled Immunoassay 2.4.2.5. Homogeneous Fluorescence Polarization (FPIA) 2.4.2.6. Homogeneous Liposome Immunoassay 2.4.3. Nonisotopic Heterogeneous Immunoassay 2.4.3.1. Enzyme-Linked Immunosorbent (ELISA) 2.4.3.2. Sandwich Immunoassay 2.4.3.3. Immunoassays using Affinity Ligands 2.4.3.4. Immunoassays with Biotin Avidin 2.4.3.5. Magnetic Immunoassay 2.5. Immunoaffinity Techniques 2.6. Immunoassays on Test Strips other Planar Structures 2.7. Characterization Interference 2.8. Future Immunological Applications Techniques