Tunable Stimuli-Responsive Polymers for Cell Sheet Engineering
作者： Anil Kumar P R , TV Kumary , Nithya Joseph
DOI: 10.5772/24297
关键词: Lower critical solution temperature 、 Biomolecule 、 Polymer 、 Cell growth 、 Substrate (chemistry) 、 Adhesion 、 Chemical engineering 、 Materials science 、 Tissue engineering 、 Proteolytic enzymes
摘要: Anchorage dependent cells require a suitable substrate or scaffold for attachment, adhesion and growth. Conventionally are detached from substrates by mechanical enzyme treatment, which can affect cell re-adhesion, viability etc. Poly (N-isopropylacrylamide) [PNIPAAm] is well known temperature sensitive polymer with LCST of 32 oC serve as good facilitating detachment without the use proteolytic enzymes. Polymer copolymers N-isopropylacrylamide [NIPAAm] been proved to be excellent treatment. The co-monomers provide functional groups that bind biological molecules form hybrid enhanced function. This chapter reviews compares different smart intelligent surfaces, method preparation, characterization techniques applications sheet engineering. PNIPAAm based surfaces able support growth then detach any chemical reagent step. These thermo responsive avoid problem associated tissue
intechopen.com
intechweb.org 
researchgate.net 参考文章(31)
I. Elloumi-Hannachi, M. Yamato, T. Okano, Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine Journal of Internal Medicine. ,vol. 267, pp. 54- 70 ,(2010) , 10.1111/J.1365-2796.2009.02185.X
Noriko Yamada, Teruo Okano, Hideaki Sakai, Fumiko Karikusa, Yoshio Sawasaki, Yasuhisa Sakurai, Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells Die Makromolekulare Chemie, Rapid Communications. ,vol. 11, pp. 571- 576 ,(1990) , 10.1002/MARC.1990.030111109
Martin T. Moran, William M. Carroll, Irina Selezneva, Alexander Gorelov, Yuri Rochev, Cell growth and detachment from protein-coated PNIPAAm-based copolymers Journal of Biomedical Materials Research Part A. ,vol. 81, pp. 870- 876 ,(2007) , 10.1002/JBM.A.31089
Thomas N. Abraham, Vidya Raj, Tilak Prasad, P. R. Anil Kumar, K. Sreenivasan, T. V. Kumary, A novel thermoresponsive graft copolymer containing phosphorylated HEMA for generating detachable cell layers Journal of Applied Polymer Science. ,vol. 115, pp. 52- 62 ,(2010) , 10.1002/APP.30538
Teruo Okano, Noriko Yamada, Minako Okuhara, Hideaki Sakai, Yasuhisa Sakurai, Mechanism of cell detachment from temperature-modulated, hydrophilic–hydrophobic polymer surfaces Biomaterials. ,vol. 16, pp. 297- 303 ,(1995) , 10.1016/0142-9612(95)93257-E
M. Heskins, J. E. Guillet, Solution Properties of Poly(N-isopropylacrylamide) Journal of Macromolecular Science, Part A. ,vol. 2, pp. 1441- 1455 ,(1968) , 10.1080/10601326808051910
Yoshikazu Kumashiro, Masayuki Yamato, Teruo Okano, Cell Attachment–Detachment Control on Temperature-Responsive Thin Surfaces for Novel Tissue Engineering Annals of Biomedical Engineering. ,vol. 38, pp. 1977- 1988 ,(2010) , 10.1007/S10439-010-0035-1
Yoshikatsu Akiyama, Akihiko Kikuchi, Masayuki Yamato, Teruo Okano, Ultrathin poly(N-isopropylacrylamide) grafted layer on polystyrene surfaces for cell adhesion/detachment control. Langmuir. ,vol. 20, pp. 5506- 5511 ,(2004) , 10.1021/LA036139F
Oh Hyeong Kwon, Akihiko Kikuchi, Masayuki Yamato, Teruo Okano, Accelerated cell sheet recovery by co-grafting of PEG with PIPAAm onto porous cell culture membranes Biomaterials. ,vol. 24, pp. 1223- 1232 ,(2003) , 10.1016/S0142-9612(02)00469-6
P. R. Anil Kumar, K. Sreenivasan, T. V. Kumary, Alternate method for grafting thermoresponsive polymer for transferring in vitro cell sheet structures Journal of Applied Polymer Science. ,vol. 105, pp. 2245- 2251 ,(2007) , 10.1002/APP.26221