Cell growth and detachment from protein-coated PNIPAAm-based copolymers
作者： Martin T. Moran , William M. Carroll , Irina Selezneva , Alexander Gorelov , Yuri Rochev
DOI: 10.1002/JBM.A.31089
关键词:
摘要: The cultivation of cells requires the use unfavorable proteolytic enzymes, which cause cell-surface modification and also need considerable optimization. Recently, with development smart polymers, research has looked to using thermoresponsive polymers as cell culture substrates. These novel surfaces allow without enzymes by utilizing phase transition property poly(N-isopropylacrylamide) (PNIPAAm). Copolymers PNIPAAm N-tert-butyl-acrylamide (NtBAm) varying ratios were synthesized solvent cast. copolymer films are coated adhesion promoters such collagen, poly-L-lysine, laminin increase their growth properties. Cell activity measured alamarBlue™ PicoGreen® assays is similar for standard tissue plastic controls. Deposition onto was characterized scanning electron microscopy (SEM) atomic force (AFM). detachment from dependent on composition not affected surface coatings extracellular matrix (ECM) proteins. results demonstrate a versatile method while eliminating digestive trypsin. © 2007 Wiley Periodicals, Inc. J BiomedMater Res
sci-hub.se 参考文章(26)
Noriko Yamada, Teruo Okano, Hideaki Sakai, Fumiko Karikusa, Yoshio Sawasaki, Yasuhisa Sakurai, Thermo‐responsive polymeric surfaces; control of attachment and detachment of cultured cells Die Makromolekulare Chemie, Rapid Communications. ,vol. 11, pp. 571- 576 ,(1990) , 10.1002/MARC.1990.030111109
Gordon Rollason, John E. Davies, Michael V. Sefton, Preliminary report on cell culture on a thermally reversible copolymer Biomaterials. ,vol. 14, pp. 153- 155 ,(1993) , 10.1016/0142-9612(93)90230-Y
Li-Chun Pan, Chih-Ching Chien, A novel application of thermo-responsive polymer to affinity precipitation of polysaccharide. Journal of Biochemical and Biophysical Methods. ,vol. 55, pp. 87- 94 ,(2003) , 10.1016/S0165-022X(02)00180-X
Masayuki Yamato, Mika Utsumi, Ai Kushida, Chie Konno, Akihiko Kikuchi, Teruo Okano, Thermo-Responsive Culture Dishes Allow the Intact Harvest of Multilayered Keratinocyte Sheets without Dispase by Reducing Temperature Tissue Engineering. ,vol. 7, pp. 473- 480 ,(2001) , 10.1089/10763270152436517
Marianne E Harmon, Mary Tang, Curtis W Frank, A microfluidic actuator based on thermoresponsive hydrogels Polymer. ,vol. 44, pp. 4547- 4556 ,(2003) , 10.1016/S0032-3861(03)00463-4
Teruo Okano, Noriko Yamada, Minako Okuhara, Hideaki Sakai, Yasuhisa Sakurai, Mechanism of cell detachment from temperature-modulated, hydrophilic–hydrophobic polymer surfaces Biomaterials. ,vol. 16, pp. 297- 303 ,(1995) , 10.1016/0142-9612(95)93257-E
Gerald Gerlach, Margarita Guenther, Gunnar Suchaneck, Joerg Sorber, Karl-Friedrich Arndt, Andreas Richter, Application of sensitive hydrogels in chemical and pH sensors Macromolecular Symposia. ,vol. 210, pp. 403- 410 ,(2004) , 10.1002/MASY.200450645
D. Weinstein, Comparison of pronase and trypsin for detachment of human cells during serial cultivation. Experimental Cell Research. ,vol. 43, pp. 234- 236 ,(1966) , 10.1016/0014-4827(66)90400-9
Teruo Okano, Noriko Yamada, Hideaki Sakai, Yasuhisa Sakurai, A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide). Journal of Biomedical Materials Research. ,vol. 27, pp. 1243- 1251 ,(1993) , 10.1002/JBM.820271005
M. Heskins, J. E. Guillet, Solution Properties of Poly(N-isopropylacrylamide) Journal of Macromolecular Science, Part A. ,vol. 2, pp. 1441- 1455 ,(1968) , 10.1080/10601326808051910