Investigation of a Sybr-Green-Based Method to Validate DNA Sequences for DNA Computing

摘要: Abstract : This project validated the generation of DNA sequences called a (n,d) code. Fifteen strands 16 nucleotides each were designed such that code strand would hybridize only with its reverse-complement and not cross- or miss-pair any other in set. The was using principles from nearest-neighbor studies thermodynamics base stacking. All possible combinations tested for their potential to miss-pair. Hybridizations representing junctions also tested. Strands cross-hybridize by measuring fluorescence over varying temperatures presence SYBR Green pools show hybridization between Watson-Crick complements is still thermodynamically favorable even when all are present. Nearly twenty 56 junction showed suitable preference direct complement did appreciably general properties modes binding I explored. experiments indicate positively-charged binds predominantly via electrostatic interactions groove binding. Its increases number pairs predictable manner.