Human adenosine deaminase. cDNA and complete primary amino acid sequence.

摘要: Abstract A previously cloned partial adenosine deaminase cDNA insert (0.8 kilobase) was used to clone additional nucleotide sequences from human HPB ALL libraries. encompassing the entire coding, and 3'-untranslated regions as well nearly all of 5'-untranslated region obtained. The complete amino acid sequence enzyme deduced protein sequencing consists 362 acids, excluding initiator Met, accounts for Mr = 40,638. Secondary structure predictions assign alpha/beta class proteins. Northern blot analysis with a probe showed mRNA be present in normal above amounts B-lymphoblasts derived deaminase-deficient patients severe combined immunodeficiency disease. Knowledge primary will pivotal further defining genetic abnormality its functional consequences expression defects.