Human adenosine deaminase and its binding protein in normal and adenosine deaminase-deficient fibroblast cell strains.
作者： P.E. Daddona , M.A. Frohman , W.N. Kelley
DOI: 10.1016/S0021-9258(19)70683-8
关键词: Tissue culture 、 AMP deaminase 、 Binding protein 、 Fibroblast 、 Chemistry 、 Radioimmunoassay 、 Adenosine deaminase 、 Molecular biology 、 Adenosine 、 Biochemistry 、 Cell culture
摘要: Reduced or absent adenosine deaminase activity occurs in all tissues of some patients with severe combined immunodeficiency disease. In this report we describe (a) a sensitive and specific radioimmunoassay for fibroblast its binding protein, (b) the importance various tissue culture media on expression deaminase, (c) levels immunoreactive compared to protein normal homozygous deaminase-deficient cell strains.
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