Immunoreactive protein in adenosine deaminase deficient human lymphoblast cell lines.
作者： D A Wiginton , J J Hutton
DOI: 10.1016/S0021-9258(19)81097-9
关键词:
摘要: Adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) was examined in human lymphoblast cell lines from normal and adenosine deaminase-deficient individuals as well heterozygous for deficiency. activity determined by a specific enzymatic assay compared to immunoreactive protein (or cross-reacting material) radioimmunoassay, order investigate mutations affecting deaminase. Two different antisera, raised goat rabbit against deaminase, had sensitivities apparent specificities when used radioimmunoassay. Rabbit antisera provided the most sensitive of enzyme, whereas antiserum detection mutant proteins. A wide range values ratio observed deficient lines, ranging near 23 times normal. The with high ratios appear contain large amounts catalytically defective or inactive protein. amount detected radioimmunoassay depends upon utilized, making much 50-fold difference. approximately half activity, thus have two. partially an unstable impaired activity. Immunoreactive visualized extracts several after electrophoresis transfer activated paper, labeling immunological probes autoradiography.
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