Adenosine deaminase-complexing protein from bovine kidney. Isolation of two distinct subunits.

摘要: We have isolated a complex of two proteins from bovine kidney that bind to adenosine deaminase immobilized on Sepharose 4B. One protein, with Mr = 110,000, comigrates both PAGE and SDS-PAGE gels complexing protein rabbit by the method Schrader et al. (Schrader, W.P., Harder, C. M., Schrader, D. K. (1983) Comp. Biochem. Physiol. B 75, 119-126). The second has 70,000. Both deaminase-Sepharose but not control resin serum albumin bound Sepharose. Based comparison partial complete denaturation appear be each other. At concentrations 0.5-1 mM increases small subunit catalytic activity 20-30%. There may some inhibition at low concentrations. designated 110,000 CP-I, 70,000 CP-II these CP.