The use of enzymes for thermal process monitoring: modification of milk alkaline phosphatase heat resistance by means of an immobilization technique
作者： Caterina Dinnella , Erminio Monteleone , Maria Felicia Farenga , Jim A Hourigan
DOI: 10.1016/S0956-7135(03)00117-8
关键词: Chemistry 、 Synthetic polymer 、 Immobilized enzyme 、 Heat resistance 、 Mole 、 Enzyme free 、 Alkaline phosphatase 、 Enzyme 、 Chromatography 、 Adsorption
摘要: Abstract Milk alkaline phosphatase (AP) was immobilized by adsorption on to a synthetic polymer that is reversibly soluble–insoluble depending the medium pH value. Immobilization experiments were conducted using different carrier concentrations. The relationship between concentration and amount of added AP expressed in terms Molar Ratio (MR) which ranged from 0.6 16.0 mol available for each mole enzyme. effect MR value activity heat stability enzyme investigated. active adsorbed depended 13.3 ± 1.4 8.0 ± 0.3 Enzymatic Units (EU) 2.0 16.0, respectively. An average D 63 ° C 130.0 ± 2.5 s calculated free form values equal 148.0 ± 4.4, 122.7 ± 5.2, 154.3 ± 7.7, 113.3 ± 6.1 77.0 ± 2.9 found samples with 0.6, 2.0, 3.8, 9.8 Over temperature 71 °C following z computed: 6.5 enzyme, 11.4 4.6 3.8
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