Interactions between interleukin-2-activated lymphocytes and vascular endothelium: binding to and migration across specialized and non-specialized endothelia.

摘要: A prerequisite for the successful immunotherapy of solid tumours with interleukin-2 (IL-2)-activated lymphocytes is their ability to home tumour tissue. Lymphocyte homing a complex process which known involve at least two independently regulated events: adhesion luminal surface vascular endothelium and subsequent transendothelial migration lymphocytes. In this study we have used an in vitro model lymphocyte employs specialized high ask whether IL-2-activated are able migrate across order leave blood vessel. Both cells monolayers cultured endothelial (HEC) were increased comparison non-activated The was mediated by function-associated antigen-1 (LFA-1) very late activation antigen-4 (VLA-4)-related pathway. LFA-1-dependent ligands on HEC other than intercellular molecule-1 (ICAM-1) VLA-4-related pathway CS1 domain fibronectin. HEC-adherent enriched natural killer (NK) CD8+ T be tumour-cytotoxic However, there no evidence cytotoxicity towards layer using syngeneic model. interaction not specific since equal numbers activated bound migrated aortic endothelium. inability discriminate between non-specialized 'flat' could responsible widespread dissemination throughout body following adoptive transfer unwanted side-effects non-involved sites.