The precursor form of the rat liver non-specific lipid-transfer protein, expressed in Escherichia coli, has lipid transfer activity.
作者： Bernadette C. Ossendorp , Teunis B.H. Geijtenbeek , Karel W.A. Wirtz
DOI: 10.1016/0014-5793(92)80374-P
关键词: Enterobacteriaceae 、 Plant lipid transfer proteins 、 Complementary DNA 、 lac operon 、 Phospholipid transfer protein 、 Gene expression 、 Biology 、 Molecular biology 、 Expression vector 、 Escherichia coli 、 Biochemistry
摘要: The cDNA encoding the precursor form or nonspecific lipid-transfer protein (pre-nsL-TP) from rat liver was cloned into expression vector pET3d. resulting plasmid transformed to Escherichia coli strain BL21(DE3). After induction bacteria with isopropyl-β-D-thiogalactopyranoside (IPTG) pre-nsL-TP purified bacterial lysate by anion exchange chromatography followed gelfiltration. From 1 culture, 6–7 mg of obtained, equal approximately 7% cytoplasmic protein. By use a fluorescence lipid transfer assay, found have activity identical mature nsL-TP.
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