Insulin induces C2C12 cell proliferation and apoptosis through regulation of cyclin D1 and BAD expression
作者： Cheng‐Juan Xiong , Ping‐Fa Li , Yang‐Liu Song , Li‐Xiang Xue , Zhu‐Qing Jia
DOI: 10.1002/JCB.24619
关键词: Myogenesis 、 Insulin 、 C2C12 、 Cell growth 、 Blot 、 Biology 、 Insulin receptor 、 Cell biology 、 Cyclin D1 、 Apoptosis
摘要: Insulin is a secreted peptide hormone identified in human pancreas to promote glucose utilization. has been observed induce cell proliferation and myogenesis C2C12 cells. The precise mechanisms underlying the of cells induced by insulin remain unclear. In this study, we for first time that 10 nM treatment promotes proliferation. Additionally, 50 100 induces apoptosis. By utilizing real-time PCR Western blotting analysis, found mRNA levels cyclinD1 BAD are upon nM/100 treatment, respectively. similar results were expressing GATA-6 or PPARα. Our identify downstream targets insulin, cyclin D1, BAD, elucidate new molecular mechanism promoting
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