Growth-inhibitory effects of the ketone body, monoacetoacetin, on human gastric cancer cells with succinyl-CoA: 3-oxoacid CoA-transferase (SCOT) deficiency.

摘要: Background Monoacetoacetin (MAA) has been used experimentally as a physiological energy source in parenteral nutrition. Succinyl-CoA: 3-oxoacid CoA transferase (SCOT) is key enzyme the metabolism of MAA. In this study, effect MAA on growth human gastric cancer cells was examined relation to SCOT expression. Materials and methods Four cell lines, OCUM-2M, MKN-28, MKN-45 MKN-74, two fibroblast lines were study. The proliferation determined by MTT assay, calculating number cells, [3H]-thymidine uptake. Cells cultured DMEM containing 10% FBS with glucose (4.5 g/L) control or g/L). mRNA expression RT-PCR. Results OCUM-2M MKN-28 significantly suppressed medium compared medium. contrast normal fibroblasts not expressed MKN-45, MKN-74 fibroblasts, but OCUM-2M. Conclusion Parenteral nutrition may provide preferential for patients some types deficiency.