Oscillating neuro-capillary coupling during cortical spreading depression as observed by tracking of FITC-labeled RBCs in single capillaries

摘要: Abstract Coupling between capillary red blood cell (RBC) movements and neuronal dysfunction during cortical spreading depression (CSD) was examined in rats by employing a high-speed camera laser-scanning confocal fluorescence microscope system conjunction with our Matlab domain software (KEIO-IS2). Following microinjection of K+ onto the surface brain, changes electroencephalogram (EEG), DC potential tissue optical density were all compatible occurrence transient depression. RBC flow single capillaries not stationary. Unpredictable redistribution RBCs at branches commonly observed, even though no change diameter apparent reported site sphincter arteriolar-venule pressure difference detected. There appeared to be slow morphological astroglial endfeet. When local neurons stunned transiently injection, velocity oscillation frequency flowing nearby started decrease. The such rectified, losing oscillatory components. Sluggish floating pertinent visualized, occasional full stops. CSD subsided, recovered original state. We postulate that depolarization blocks signaling via low-shear plasma viscosity increases channels, complex interaction buffy coat on wall resistance. Then, when subsides function is recovered, normal physiological conditions are restored.