Core-lipid A on the K40 polysaccharide of Escherichia coli O8:K40:H9, a representative of group I capsular polysaccharides.
作者： Klaus Jann , Thomas Dengler , Barbara Jann
DOI: 10.1016/S0934-8840(11)80006-X
关键词: Heptose 、 Antiserum 、 Polysaccharide 、 Biology 、 Lipopolysaccharide 、 Glucuronic acid 、 Glucosamine 、 Lipid A 、 Biochemistry 、 Galactose
摘要: Summary From the capsular K40 polysaccharide of E. coli O8: K40: H9, a fraction was obtained by gel permeation chromatography which in SDS-PAGE exhibited ladder-like pattern characteristic lipopolysaccharides. In Western blots, this reacted with K40-specific antiserum but not an O8-specific antiserum. It contained, addition to constituents (glucuronic acid, glucosamine and serine), glucose, galactose, heptose, KDO. Mild acid hydrolysis liberated lipid moiety chemical analysis characterized as A. these results, we conclude that H9 is part bound core The significance finding discussed.
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