Process for producing gene products of plasmid having temperature dependent plasmid copy number

作者: Bernt E. Uhlin , Kurt Nordstrom , Soeren Molin

DOI:

关键词: PlasmidGeneCloning vectorBacteriaPlasmid preparationRecombinant DNAMolecular biologyPlasmid copy numberBiologyT-DNA Binary system

摘要: Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a which shows controlled constant copy number at one temperture and much higher or totally uncontrolled different temperature. The may be recombinant DNA technique using cloning vector showing the temperature dependent pattern.

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