作者: Bernt E. Uhlin , Kurt Nordstrom , Soeren Molin
DOI:
关键词: Plasmid 、 Gene 、 Cloning vector 、 Bacteria 、 Plasmid preparation 、 Recombinant DNA 、 Molecular biology 、 Plasmid copy number 、 Biology 、 T-DNA Binary system
摘要: Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a which shows controlled constant copy number at one temperture and much higher or totally uncontrolled different temperature. The may be recombinant DNA technique using cloning vector showing the temperature dependent pattern.