Parasite-specific antibody profile in the aqueous humor of rabbits with ocular toxocariasis.

摘要: Abstract Human ocular toxocariasis is diagnosed using ophthalmologic and immunologic examinations. Many researchers have suggested that intraocular parasite-specific antibody levels are indicative of toxocariasis, but little known about the time course changes in these levels. We therefore investigated anti-Toxocara canis profile aqueous humor an animal model toxocariasis. intravitreally injected T. larvae into right eye 4 rabbits; 2 rabbits were orally administered eggs. collected serum, humor, tear samples weekly determined serum anti-T. immunoglobulin (Ig)G, IgA, IgM, IgE antibodies IgG level by enzyme-linked immunosorbent assay (ELISA). The severity vitreous opacity (measured optical density [OD]) changed concordantly larvae-injected eyes; OD exceeded 0.1 from 2–4 weeks after infection remained elevated during active inflammation. However, also 6 out 8 eyes without both groups, low 1 with live intravitreal larvae. In contrast, IgM increased all rabbits, regardless presence Vitreous occurred infected eyes, significant histopathological evidence retinal damage was not detected. Thus, besides larvae, some other factors host may be required for development lesions.