Metabolic flux analysis of hydrogen production network by Clostridium butyricum W5: Effect of pH and glucose concentrations

摘要: Abstract Fermentative hydrogen production by strict anaerobes has been widely reported. There is a lack of information related to metabolic flux distribution and its variation with respect fermentation conditions in the system. This study aimed get better understanding network conduct analysis (MFA) fermentative recently isolated Clostridium butyricum strain W5. We chose specific growth rate as objective function used H2 criterion evaluate experimental results silico MFA. For first time, we constructed an model for anaerobic glucose metabolism C. W5 assistance modeling program MetaFluxNet. The was network, fractional response variations initial concentration operational pH. MFA suggested that pH more significant effect on yield compared concentration. useful tool provide valuable optimization design process.