Isolation and identification of the dominant Lactobacillus in gut and faeces of pigs using carbohydrate fermentation and 16S rDNA analysis.

摘要: The gut contents and faeces of 9 adult pigs 387 colonies growing on MRS culture (selective for Lactobacillus) were sampled to isolate identify Lactobacillus species present. results showed that the number bacteria increased from anterior posterior decreased in (e.g., stomach, 4.04 x 10(5) cfu/g; small intestine, 9.77 10(7) large intestine 3.85 10(11) faeces, 1.22 cfu/g). Fifty-two lactobacilli-like selected basis their size Gram staining, used fermentation 11 carbohydrates, which 12 lactobacilli 16S rDNA analysis. result ruminis was dominant pigs. identification most strains estimated by 98-99% accurate.